Monolithic Chromatography and its Modern Applications

High-performance liquid chromatography has become the predominant global separation technique over the past four decades. However, the recent development of an alternative column packing material – a one-piece porous solid known as a ‘monolith’ – has transformed the field. Monoliths have attracted a large amount of interest due to the enhanced speed and thoroughness with which they can separate complex mixtures of biological molecules.

Monoliths are porous rod structures characterised by mesopores and macropores. These pores provide monoliths with high permeability, a large number of channels and a high surface area available for reactivity. The backbone of a monolithic column is composed of either an organic or inorganic substrate and can easily be chemically altered for specific applications. Their unique structure gives them several physico–mechanical properties that enable them to perform competitively against traditionally packed columns.

In this book, global experts comprehensively describe this developing field. Part I covers the synthesis and characterisation of the columns and Part II dicusses their numerous applications.

This volume will be of interest to those already using monolithic columns, as well as those who are new to the area and would like to know more about this technique.

Part I Synthesis and Characterisation of Monolithic Columns
Chapter 1 Affinity Monolith Chromatography: Principles and Recent Developments
Michelle J. Yoo and David S. Hage

Chapter 2 Characterisation of Pore Structure and Its Impact on the Chromatographic Properties of Organic Polymer Monolithic Capillary Columns
Pavel Jandera and Jiri Urban

Chapter 3 Silica-based Porous Monoliths: Synthesis, Characterisation and Applications in Chromatographic Separation
Hua Zhong and Qihua Yang

Chapter 4 Preparation and Characterisation of Organic Polymer Monolithic Capillary Columns
Pavel Coufal, Zuzana Bosakova, Jakub Sirc, Vera Pacakova and Karel Stulık

Chapter 5 Ring-opening Metathesis Polymerisation- derived Capillary Monoliths: Versatile Analytical Tools for Modern Biomedical Research
Frank M. Sinner and Christina Gatschelhofer

Chapter 6 Monolithic Cryogel Beds Prepared Under Varying Freezing Temperatures and Their Application in the Isolation of Nucleotides
Kejian Yao, Junxian Yun and Shaochuan Shen

Chapter 7 Organic Monoliths as Stationary Phases in Chromatography
Andreas Greiderer, S. Clark Ligon Jr., Christian W. Huck and Gunther K. Bonn

Part II Applications of Monolithic Columns
Chapter 8 Applications of Monolithic Chromatography in Pharmaceutical Analysis
Debby Mangelings and Yvan Vander Heyden

Chapter 9 Monolithic Columns in Microscale Separations
Ren’an Wu and Hanfa Zou

Chapter 10 Monolithic Ion Exchange Phases for the Separation of Small Inorganic and Organic Ions
Brett Paull and Damian Connolly

Chapter 11 Monolithic Column Chromatography Coupled with Chemiluminescence Detection
Paul S. Francis and Jacqui L. Adcock

Chapter 12 Fast Separations of Phytochemicals by High-performance Liquid Chromatography Using Monolithic Columns
Mauricio A. Rostagno and Ana Villares

Chapter 13 Monolithic Stationary Phases for Affinity-based Separations
Panagiotis Manesiotis and Georgios Theodoridis

Chapter 14 Immobilisation of Enzymes on Monolithic Matrices: Applications in Drug Discovery
Vincenza Andrisano and Manuela Bartolini

Chapter 15 Applications of Monolithic Chromatography in Support of Pharmaceutical Development of Drug Substances and Formulations
Naijun Wu and Zhong Li

Chapter 16 Use of Monolithic Material as On-line Extraction Support for High-throughput Liquid Chromatography Tandem Mass Spectrometry Analysis of Biological Samples
Raymond Naxing Xu, Grace E. Kim, Maria P. Metchkarova, Phillip Vaca, Matthew J. Rieser and Tawakol A. El-Shourbagy

Chapter 17 Convective Interaction Media Monoliths for Separation and Purification of pDNA and Viruses
Matjaz Peterka, Petra Kramberger and Ales Strancar

Chapter 18 Analysis of Methylated Arginines and Amino Acids Using a Monolithic Silica Column
Makoto Tsunoda

Chapter 19 Applications of Monolithic Columns in the Analysis of Illicit Drug Samples
Kim McFadden and Brian Carney

Chapter 20 Applications of Monolithic Phases in Fundamental Studies of Vegetable Proteins and Food Analysis and Technology
J.M. Saz and M.L. Marina

Chapter 21 Toxicological Analysis Using Monolithic Silica Spin-column Extraction and Chromatographic Separations
Takeshi Saito, Akira Namera and Akihiro Nakamoto

Chapter 22 Amphiphilic Acrylamide-based Continuous Beds in Capillary Electrochromatography
Olga Kornysova, Audrius Maruska and Ute Pyell

Index

Dr. Perry G. Wang received his BS degree in chemistry from Shandong University, China. He earned his MS and PhD degrees in environmental engineering from Oregon State University, USA.

He is currently a Research Chemist in the Office of Regulatory Science, Center for Food Safety and Applied Nutrition, US Food and Drug Administration (US FDA). His activities include analytical method development and validation for drugs and constituents in foods and cosmetic products using advanced instrumentation. His expertise focuses on high-throughput drug analysis by LC/MS/MS for the pharmaceutical industry.

Dr. Wang has been invited to prepare, organise and preside over symposia for the Pittsburg Conference (PittCon) and American Chemistry Society (ACS) Annual Meetings. He has been an invited speaker at numerous international conferences including PittCon, the Federation of Analytical Chemistry and Spectroscopy Societies (FACSS) Meetings, the Beijing Conference and Exhibition on Instrumental Analysis (BCEIA) and the International Symposium on Chemical Biology and Combinatorial Chemistry (ICCBCC). He also has been invited to teach short courses on high-throughput method development for drug analysis by LC/MS/MS at PittCon, ACS Annual Meetings and the Eastern Analytical Symposium (EAS).